The F-BAR domain of srGAP2 induces membrane protrusions required for neuronal migration and morphogenesis.

Bibliographic Collection: 
MOCA Reference, APE
Publication Type: Journal Article
Authors: Guerrier, Sabrice; Coutinho-Budd, Jaeda; Sassa, Takayuki; Gresset, Aurélie; Jordan, Nicole Vincent; Chen, Keng; Jin, Wei-Lin; Frost, Adam; Polleux, Franck
Year of Publication: 2009
Journal: Cell
Volume: 138
Issue: 5
Pagination: 990-1004
Date Published: 2009 Sep 4
Publication Language: eng
ISSN: 1097-4172
Keywords: Animals, Carrier Proteins, Cell Movement, Cerebral Cortex, Mice, Neurogenesis, Neurons, Pseudopodia

During brain development, proper neuronal migration and morphogenesis is critical for the establishment of functional neural circuits. Here we report that srGAP2 negatively regulates neuronal migration and induces neurite outgrowth and branching through the ability of its F-BAR domain to induce filopodia-like membrane protrusions resembling those induced by I-BAR domains in vivo and in vitro. Previous work has suggested that in nonneuronal cells filopodia dynamics decrease the rate of cell migration and the persistence of leading edge protrusions. srGAP2 knockdown reduces leading process branching and increases the rate of neuronal migration in vivo. Overexpression of srGAP2 or its F-BAR domain has the opposite effects, increasing leading process branching and decreasing migration. These results suggest that F-BAR domains are functionally diverse and highlight the functional importance of proteins directly regulating membrane deformation for proper neuronal migration and morphogenesis.

DOI: 10.1016/j.cell.2009.06.047
Alternate Journal: Cell